The goal of this study was to investigate a possible association of chemokine 12 plasma level  and its association to ITP , This was a prospective, observational, case-control study which was conducted on 60 patients with ITP with age ranging from 1 -18 years old together with 90 healthy controls. The plasma level of chemokine 12 in pediatric ITP patients and healthy controls were determined using enzyme linked immunosorbent assay (ELISA) The detection limit for this assay was 1.5 pg per ml.  Patients were subjected also to lab investigation in the form of CBC and BM aspirate and There was a high plasma level of chemokine 12  in cases than controls (p value <0.05). Furthermore, cases with high plasma level of chemokine 12 were more susceptible for steroid dependency than controls  (p < 0.05) otherwise, no significant differences between plasma level of chemokine 12 among cases as regard clinical picture , history data , ITP course.