Background: Anal fistula and anal sepsis are the part of spectrum of anorectal suppurative diseases, A fistula-in-ano is a hollow tract lined with granulation tissue connecting a primary opening inside the anal canal to a secondary opening in the perianal skin through which an abscess has been drained or has spontaneously ruptured. Aim of the Work: To compare injection of histoacryle and irrigation of silver nitrate in treatment of low non branching anal fistula. Patients and Methods: This study was held in Beni-Suief university hospital, at the general surgery department from October 2019 to June 2020 on 50 patients that divided into 2 groups randomly selected (SN group and Histoacryle group). Results: There is a significant difference found between two groups in favor for SN. As silver nitrate is much less expensive with a lower recurrence rates, while histoacryle is more expensive with higher recurrence rates. Conclusion: Both silver nitrate and histoacryle can be used in treatment of low non branching anal fistula effectively with no evident complications and good success rate specially for patients refusing surgery and for patients with high risk for anesthesia, favoring SN injection as it has lower recurrence rates and cheaper cost in comparison with HA injection.