The aim of the present study was to identify the incidence of sepsis among neonates admitted at Beni-Suef University Hospital during the period of the study, to identify different organisms responsible for sepsis and the serum level of CD14 (presepsin) was investigated as a biomarker of sepsis. All neonates suspected with sepsis during the time of the study admitted in neonatal intensive care unit at Beni-Suef University Hospital were screened for sepsis by blood culture and presepsin levels were investigated. The incidence of sepsis was 32.4%. Presepsin marker had a significant role in prediction of presence of sepsis, at a cut off value of 2300 or more of presepsin, it can predict the presence of sepsis with sensitivity of (94.29%), specificity of (88.6%), positive predictive value (89.2%) and negative predictive value (93.9%)