The goal of this study is to detect a relationship between advanced glycation end-products and the pathogenesis of vitiligo in diseased patients as compared to normal control persons using Enzyme-Linked Immunosorbent Assay (ELISA) technique on tissue skin biopsy. The present study included 30 Egyptian vitiligo patients, their age ranged from (20 to 50 years). They were recruited from Dermatology outpatient clinic at Beni-Suef University hospital. Thirty unrelated apparent healthy controls with similar demographic (matched age and sex) were taken.The studied subjects were divided into two groups as follows, Group I: (n = 30) vitiligo patients and Group II: (n = 30) unrelated apparent healthy controls. Four mm punch skin biopsy will be taken from patients( vitiligo lesion) & control and it will be kept in lysis solution for the stability of the studied parameters and will be kept frozen at -80 Celsius till analysis of  advanced glycation end-products by ELISA. The result of the study showed that expression of AGEs in lesional skin of Vitiligo patients was significantly higher as compared to normal control persons.  Also reveled moderate negative correlation between the expression of Advanced Glycation end-Products (AGEs) in lesional skin of vitiligo patients and Vitiligo disease activity (VIDA) score of the studied cases and that confirms that AGE levels were, instead, significantly higher in vitiligo patients with older lesions.