The goal of this study is to assess various aspects of blood transfusion services and blood safety and assure the adherence of the studied blood banks to the WHO global blood safety guidelines in Beni-Suef blood banks. This cross-sectional study was conducted in 2018 by in depth interview in the University hospital blood bank and the regional blood bank of Beni-Suef governorate. The officially recognized and the nationally coordinated blood transfusion service (BTS) found in both banks. There is a national blood policy and a national BTS management committee in both banks. There is a medical director responsible for the BTS and a national quality system in both banks. There is a mechanism for calculating total BTS costs in both banks. The approximate cost in US$ of producing one unit of whole blood is approximately 10 dollars (about 170 LE) and a system of stock control in both banks. Both blood banks are working under the WHO guidelines for global blood safety.