Health professionals are a direct source of medical information to the public. Hence, it is crucial that their knowledge is accurate and aids in building awareness. Aim: Increase the knowledge and ability of female nurses to disseminate proper information about breast and cervical cancer to the population. Methodology: An observational cross-sectional study was conducted from April 2017 to January 2018, among 350 nurses working in Beni-Suef university hospital, El Salam private hospital, rural and urban PHC units were surveyed by a self-administered questionnaire to measure their knowledge about cancer cervix. Results: The majority of nurses (76.6%) had an optimal knowledge about cancer cervix with optimal knowledge score about all domains except general knowledge which was suboptimal=57.8%. The knowledge score was significantly affected by some socio-demographic factors. The main source of information about cancer cervix was colleagues by (70.5%) of nurses. Conclusion: There is a need for educating nurses about cancer cervix with giving priority to improve cancer cervix content in the educational nursing curriculum with the development of periodic workplace training courses for nurses and continuous professional education programs that focus on Pap smear for health care workers.