This study focused on demonstrates improving the antibacterial activity and white resist printing for viscose fabrics in one step. Chitosan is the deacetylated derivative of chitin; it is a functional biopolymer which can be safely used for textile wet process. The efficacy of resisting agent was studied in comparison with standard unprinted illuminated fabrics. Results show that an improvement in antibacterial activity and dye blocking. In all cases, the reprinted fabrics were more oversensitive towards Gram positive bacteria than Gram negative bacteria due to bacteria structure. This method can be suitable for eco-friendly resist printing on viscose fabrics.