These experiments were carried out at (Prof. Dr. Abdel Fattah Helmy Belal) plant tissue culture laboratory, Faculty of Environmental Agricultural Sciences - Arish University, North Sinai, Egypt, during the period from 2018 to 2021. The aim of this study was to investigate the effect of some factors affecting micropropagation of Paulownia hybrid (Paulownia elongate × Paulownia fortunei) to cover the cumulative demand on this plant. Different media (MS, DKW, WPM and B5), explant types (shoot tip and nodal segment), cytokinin types (Kin, BA and 2Ip), gelling agent types (guar gum, gellan gum, gelrite, corn starch, wheat starch, and locust bean), and IBA concentrations (0.0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0) mgl^-1, were studied to identify the most effective treatments for in vitro micropropagation of Paulownia hybrid (Paulownia elongate × Paulownia fortunei). Results clarified the superiority of nodal segment over shoot tip, and superiority of MS medium over all other tested media. The addition of BA (3 mgl^-1) to MS medium was the most effective treatment for shoot proliferation compared with all other tested cytokinins. At the multiplication stage, results showed that the most effective gelling agent were agar alone and its combinations with gillan gum at 2 – 6 gl^-1.  During rooting stage, using of IBA at 1mgl^-1 was more effective on increasing root number and root length (cm) compared with control treatment.