Sixteen new lines of tomato (F5) were selected from six F2 generations, which exhibited high homogeneity based on estimated CV % values for plant height, number of leaves/plant, hardness, shape index, TSS% and Vitam. C content during successive four seasons from 2017/2018 to 2020/2021 in the Experimental Farm, Faculty of Environmental and Agriculture Sciences, Arish University, North Sinai under open field conditions. The experimental work was conducted using a randomized complete block design with three replications. A highly significant differences were observed among the selected lines for all studied characters. Lines that showed the most vigorous growth were DAN-2-2, DAN-3-4, DAN-3-5, 6130-1-1, 6130-3-2, 6130-3-4, 3017-2-1, 3017-2-6, 783N-1-4, TYG-1-3, TYG-2-1, TYG-2-1, TYG-4-1 and KIS-N-2-1. For Mean performance all plants in the lines 6130.1.1, 6130.3.4, 3017.2.1, 3017.2.6, TYG-1-3 and KIS-N-2-1 were resistance to Tomato yellow leaf curl virus. Two lines recorded highest values for total yield, 3017.2.1 and 3017.2.6, while 6130.3.2 and 6130.3.4 where the best for avrage fruit weight.