Biofumigation is the practice of using Brassica green manure plants with high content of glucosinolates which enzymatically hydrolyses to nematicidal isothiocynate (ITCs) compounds. Brassica species as well as cultivars differ in both type and concentration of glucosinolate (GSLs). Under North Sinai conditions, however, the highly effective amongst Brassica species that can be effectively used are not documented for specific target yet. In this study two successive field trials were conducted using tomato (cv. Elysa) aimed at  evaluating and determining the efficacy of four  Brassica species (Fodder radish (Raphanus sativus) TERRANOVA H-4-169/0300), Canola (Brassica napus) (cv. Serow 4), Mustard  (Brassica alba) (local commercial cultivar) and Rocket Salad (Eruca sativa((local commercial cultivar) in comparison with vydate (as positive control) and untreated (as a negative control) in suppressing root knot nematode population (Meloidogyne incognita), reproductive factor and galling index as studied nematode parameters. After 2 weeks of tomato seedlings transplanting both root knot nematode population and reproductive factor were recorded. After termination of the two experiments (12 weeks) nematode parameters and tomato plant growth attributes were recorded. Fodder radish was significantly the most effective brassica green manure in controlling root knot nematodes (Meloidogyne incognita) compare to other three studied brassicas. All studied parameters of fodder radish green manure were comparable with that of vydate. The study recommends that biofumigation methods using fodder radish green manure can be used through integrated pest management program (IPM) for both conventional and organic agriculture tomato production systems.