Canola plant (Brassica napus L.) is an important oil seed crop, which takes the second place among oil crops after soybean which contributes as edible oils over the world. This study was conducted to investigate the effect of five levels of nitrogen fertilizer T1=organic fertilizer, T2= 37.5 Kg N/fed, T3= 75 kg N/fed, T4= 112.5 kg N/fed and T5= 150kg N/fed and six canola genotypes (Serw4, Serw6, Pactol AD201,Tobas, and Silvo) on viscosity, specific gravity, refractive index, fatty acid composition, acidity, saponification, peroxide, iodine values ,Unsaponifiable matter(USM), and followed its oxidative stability at 60^oC for 30  days. The results showed that increased of nitrogen fertilizer rate leading to decrease led to decreases d viscosity, specific gravity, acid, saponification and  peroxide values, while  increasing  unsaponifiable  matter. 150 kg N/fed was the optimum nitrogen dose for realizing the lowest  acid and peroxide values for Silvo genotype, While this dose caused a negligible differences in iodine value and refractive index. In terms of genotype differences, the acid and peroxide  value results revealed that Silvo genotype  was the lowest , while Pactol variety was the highest one. All chemical properties improved that Silvo genotype gave the highest stability during storage at 60^o C compared to organic fertilizer.