"Kalamata" olive trees (Olea europea L.)about 10-years-old of nearly moderate vigorand productivity grown in sandy loam soil were selected. The trees were planted at 6×7 m apart under drip irrigation system. All trees received regularly the annual horticultural practices as usually adopted in the orchards. The four organic fertilizer sources i.e., fish scrap 2.5 Kg/ tree/ year, goat manure 16.8 Kg/tree/year, chicken manure7.8 Kg/tree^/year and olive pomace 8.5 Kg/tree/year; were applied with combination of biofertilizers Nitrobein 150 g/tree/year and Protamine^® 1.5% and dissolving in one liter of water then added to the soil at three times, at 70% full-bloom, after fruit set and a month later. According to the results obtained in this study, it is possible to recommend the use of organic fertilization, especially fish scrap, through the addition of bio-fertilization using Nitrobein with amino acid Protamine at a concentration of 1.5% combined. This treatment improved the vegetative growth and the leaf mineral content of olive trees to protecting the environment from the problems of pollution resulting from mineral fertilization as well as the use of environmental waste.