Fifty grams of Moringa seeds were ground and then sieved. The solvent methylene chloride was added to the fine powder. The obtained solution was submitted to column chromatography analyzed. Each output was separated and identified by Fourier Transform Infrared spectroscopy (FTIR), ¹³Carbonand Nuclear magnetic resonance spectroscopy. The obtained substances were tested against each of the adult females of the two spotted spider mite Tetrancus urticae (Koch) and the cotton mealybug Phenacoccus solenopsis Tinsley. The efficiency effects of the obtained compounds were evaluated after 24 hours after application. No effect for all the tested compounds was recorded except for Rhamnosyloxy-benzyl isothiocyanate. The results showed that the values of LC₂₅, LC₅₀ and LC₉₀ were 150, 388 and 2370 ppm, respectively after application for the two spotted spider mite T. urticae, whereas LC₂₅, LC₅₀ and LC₉₀ were found to be 251.14, 839.89 and 8326.2 ppm after 24 hours of application, respectively on the cotton mealybug P. solenopsis.