These experiments were carried out at the apiary of honeybee research center of Faculty of Environmental Agricultural Sciences, Arish University, North Sinai, Egypt. To study some factors that affecting on the honey bee venom collection (bee race, season of the year and supplemental protein nutrition) on collected venom quantity .The results showed that the Italian hybrid gave the largest quantity of venom. Spring and summer are the best seasons for collecting the largest quantity of the bee venom. The pollen grain and dried Moringa leaves diets treatments gave the best results for bee venom production comparing with the other treatments (mixture of soybean flour + pollen grains and sugar solution).