Experiments were conducted in the apiary of Honey Bee Research Center. A field experiment was carried out during 2014 and 2015 seasons, at the Experimental Research Station of the Agriculture Research Center, El-Arish, North Sinai, to study the impact of different sources of nutritive protein on honey bee worker venom gland secretion. The aim of this study is to determine the effects of some factors that could increase the productivity of honeybee venom. This investigation discusses improving the production of the venom from honeybee's colonies by using the electrical impulses technique. This study includes several essential factors, such as using different types of nutrition proteins 1:1 W: W of honey bee to beer yeast or soya beans or natural pollen grain, respectively. Results indicated that the natural pollen grain is the best because it is mainly provide structural elements of muscles, glands and other tissues.