Almond (Prunusdulcis) is one of the most important nut fruits worldwide and its seed has been recognized as a health nutrient supply for human consumption. In this study, as well as their genetic diversity and natural variations using SRAP marker, aiming at exploiting valuable traits for breeding new cultivars. Our study of identified Molecular evaluation of genetic diversity at the DNA level for relationships and population structure among 12 almond genotypes, Identifying alleles (s-alleles) of the genre of self-incompatibility and self-compatibility gene,  and preservation of these important resources, the wild distribution of almond and closely related species. A total of 48 bands ranging from 100 to 2000 bp were detected using the 3SRAP primer combinations in the twelve populations (Table 4). The numbers of bands per primer combination ranged from 6 to 21, with an average of 11.57.161 (99.35%) were polymorphic, and the percentage of polymorphic bands produced by each primer ranged from 100%.