In this study the efficacy of three chemical fungicides as well as five different biological control agents were tested in vitro against several isolates of Fusarium oxysporum from wilted date palm trees grown in North Sinai. The three commercial fungicides were: Tachigaren 30% SL, Rhizolex-T 50% WP and Dithane M-45 80% WP. Whereas the biological control agents included three fungal species namely: Trichoderma album, T. harzianum and T. strigosum. As well as the two bacteria: Bacillus megaterium and B. subtilis. Tachigaren was the most effective fungicide in reducing the fungal growth of all tested isolates of
F. oxysporum. Rhizolex T-50 ranked second while Dithane M-45 was the least effective fungicide in reducing the mycelial growth of the tested isolates of F. oxysporum in vitro. Trichoderma harzianum was the most effective biocontrol agent in inhibiting the mycelial growth of all tested F. oxysporum isolates followed by T. album and then T. strigosum. On the other hand, Bacillus megaterium and Bacillus subtilis were generally non effective in reducing mycelial growth of the tested isolates of F. oxysporum in vitro.