A field study was carried out during summer seasons of 2013 and 2014 at the Experimental Farm, Faculty of Environmental Agricultural Sciences, Arish University, Egypt. It aims to study the effect of organic fertilizer sources (cow manure "CM"; chicken manure (ChM); pressed olive cake (POC); compost "Comp"} and two bio-fertilizer sources (Bio-1 and Bio-2) plus without biofertilizer on summer squash (Cucurbita pepo L.) cv “Askandrany" under EL-Arish conditions. The experiment included 12 treatments and the experimental design was split plot design with three replications. Organic fertilizers were randomly distributed in the main plots (four sources), while, sub plots contained three bio treatments (without bio, Bio-1 and Bio-2). The obtained results indicated that cow manure treatment gave the highest values of number of fruits/plant, mean fruit weight, yield/plant and yield/fed., also, cow manure x Bio-1 treatment gave the highest value in case of average fruit weight/plant, while, cow manure x Bio-2 treatment gave the superior values in cases of number of fruits/plant, yield/ plant and yield/fed., in both seasons. Results of the interaction between organic and bio fertilizers, chicken manure and Bio-1 produced the highest value of TSS%.