A field experiment was carried out at the Experimental Farm, Faculty of Environmental Agricultural Sciences, Rafah, North Sinai Governorate, Suez Canal University during the two successive winter seasons of 2011/2012 and 2012/2013; to study the response of 5 canola cultivars (Serw4, Pactol, Sakha1, Sakha2 and Sakha3) to five biofertilization treatments B₁ (100% mineral + 0% biofertilizer), B₂(75% mineral + 25% biofertilizer), B₃ (50% mineral + 50% biofertilizer), B₄(25% mineral + 75% biofertilizer) and B₅ (100% biofertilizer) on yield and yield components. The results showed that Serw4 cv., surpassed the other cultivars in plant height, No. siliquae/plantat the 2^nd season. Pactol surpassed the other cultivars in 1000-seed weight at 1^st season but Sakha₁ surpassed other cultivars in 1000-seed weight at 2^nd season. Application of B₁ treatment, significantly increased, plant height, No. racemes/plant, No. siliquae/plant, while B₃ and B₄ treatments gave the heighst 1000-seed weight at both seasons. Generally, it could be concluded that Serw₄ cv. fertilized by 100% mineral and 0% biofertilizer gave the heights yield and could recommended under North Sinai conditions.