Experiments were conducted in the Tissue Culture Laboratory, Faculty of Environmental  Agricultural Sciences (FEAS), EL-Arish, Suez Canal University during the period from 2011-2014. The resent study to aimrd determine the optimen on hermone concentrations and light intensities potato microtubers by tissue culture technique of two potato cultivars, "Spunta" and "Diamant". production of microtubers in vitro where two experiments were conducted to study the effect of: (A) 6- benzyl adenine concentrations with light intensities on multiplication and microtubers production, (B) kinetin concentrations with light intensities on multiplication and microtubers production, addition of BA at rate 1.0 mg/l was recorded with both cultivars and gave the best results for all microtuberization parameters for all studied traits in the first experiment. Concerning the second experiment, "Diamant" cv. with 1000 lux had the highest record values; the results showed that light intensity 1000 lux with 4.0 mg/l of kinetin have significant effects during 60 and 90 days on multiplication and microtuber production for all studied traits.