This study was carried out in Plant Tissue Culture Laboratory in Faculty of Environmental Agricultural Sciences (FEAS) El-Arish, North Sinai, Suez Canal University (SCU) during the period from 2012 to 2014. The objective of this study was to propagate Artemisia judaica L. by using tissue culture technique. The results indicated that BA at 1.0 mgl^-1 with NAA 0.1 mgl^-1 gave the highest values of number of axillary shoots/explant (2.0 shoots/explant), axillary shoot length and highest number of leaves/shoot. Also, MS media produced the highest number of axillary shoots/explants and axillary shoot length followed by Chee and Pool medium. The highest number of leaves/shoot was recorded with MS media supplemented with BA at 1.0 mgl^-¹ combined with 0.1 mgl^-¹ NAA. Addition of casein hydrolysate at 2.0 gl^-1 combined with BA at 1.0 mgl^-1 + NAA at 0.1 mgl^-1 increased No. of axillary shoots and No. of leaves/shoot. Callus fresh weight was increased with the combination of 2.0 mgl^-1 2,4-D and 0.2 mgl^-1 kinetin after four subcultures. MS basal medium supplemented with 1.0 mgl^-1 NAA produced increase in No. of roots and No. of leaves/plantlet. Addition of IBA at 1.5 mgl^-1 recorded the highest values of all rooting parameters. Plantlets were successfully acclimatized in mixture of peat moss and sand (3:1, v/v).