This study aimed to identify the effect of using Kinesio tape on the neuromuscular activity of quadriceps muscle after the rehabilitation program of the knee joint. Ten players participated in present study (age: 26.20 ± 1.69 years; body mass: 77.10 ± 4.48 kg; height: 172.30 ± 5.46 cm). The EMG signals were stored at a sampling frequency of 1000 Hz and digitized using a 16-bit analogue to digital (A/D) converter. EMG data were processed using ProEMG software (Myon 320, Schwarzenberg, Switzerland). Raw EMG data was band-pass (20 Hz- 450 Hz) applying a Butterworth filter. The signals were preprocessed using full-wave rectified and a linear envelope obtained using the Root Mean Square (RMS) approach with a window size of 300 ms. Data was normalised to the peak muscle activity value over each attempt (%MAX). Descriptive statistics were reported as means and standard deviations (mean ± Std. Deviation). The normality of the data was analyzed using the Shapiro-Wilk test. Independent T-test was used to detect significant differences and compare the mean of each variable between single-leg squat and balance tests. The statistical analysis was performed using IBM SPSS software Statistics v21 SPSS Inc., USA) with significance level defined as p < 0.05 for each test. The results of the present study showed that applying KT around the knee joint after rehabilitation program does not promote immediate changes or effects on the lower limb muscle activities of (VL, RF, VM, BF, ST, L_GAST, and M_GAST