ABSTRACT This study was aimed to determine the effect of adding quinoa seeds powder (QSP) on biological, biochemical and histological parameters of albino male rats as well as preparing functional biscuit supplemented with quinoa powder. The results of QSP in the current study show that DPPH radical scavenging activity (%) was gradually increased with the increasing the concentration from 1.5 to 10. Whereas, IC₅₀ was recorded 4.39. Wheat flour was substituted with quinoa powder at a rate of 10,15 and 20% in tested biscuit. The panel test revel that 20% quinoa biscuit was recorded the highest general acceptability values by the panelists. So, this quinoa percentage was used in the biological experimental design. Twelve male rats weighting 120±10g were divided into two main groups (6 each). The first group was fed on basal diet and the second group was fed on basal diet containing 20% QSP. Nutritional, biochemical and histological parameters revel that feed intake, body weight, feed efficiency ratio, AST, ALT, Creatinine(Cr), TG, TC, LDL and VLDL were insignificant decreased compared with control group. Whereas, urea, Blood Urea Nitrogen (BUN) and HDL were insignificant increased compared with control group. The results of histological examination for liver and kidney were similar to those of the previous studies. According to these findings quinoa may be used in several food products and might has a beneficial food property.