ABSTRACT
Microorganisms capable of degrading cellulose present in rice straw was isolated from wastewater samples and identified as Aspergillus  niger MT809753 by 18S rRNA. In the present study various cheap agronomic cellulosic wastes as (cotton seed husks, barley straw, rice straw and maize straw) were utilized as crude inducers for the cellulase enzyme production , cellulose activity was measured by dinitrosalicylic acid (DNS) method.  The highest cellulase enzyme production was obtained by fungal isolate Aspergillus  niger MT809753within 24 h (0.532 IU ml ^-1) using rice straw. Plackett-Burman design was used as conventional method for statistically screening of different variables. Seven variables from nine had influence on cellulase production with high confidence levels. Cellulase production became 1.08 IU ml ^-1  after using response optimization. A bench scale study was performed to examine   paper industry wastewater treatment efficiency by Aspergillus Niger MT809753. Results reveal that organisms have proved their bioremediation potency in treatment of paper industry effluent. Our goal is to obtain local isolates from fungi having a high ability to produce the cellulase enzyme, as well as developing an effective treatment processes to get rid of environmental cellulosic pollution and utilization of cellulosic wastes as cheap carbon sources.