ABSTRACT A pots experiments were carried out at the experimental Farm of Kafer El-Hamam Agric. Res. Station, El Sharkia Governorate, Egypt in season 2019 to evaluate using rhizo-filtration for biofortification of oil canola (Brassica napus L.) cv. Serw 4 with selenium. Canola plants which planted in water culture were treated with selenium (as sodium selenate “Na₂SeO₄" form) at a concentration of 0 (control), 1, 1.5 and 2 mgL^–1 in addition to control. The results showed that there were statistically significant differences between the treatments on the studied attributes with superiority canola plants treated with 2 mg Se L^–1 to other treatments. The relative increase in total dry weight of canola were 11.36, 21.99, 31.04% of canola plants treated with 1, 1.5 and 2 mg Se L^–1, respectively comparing with control. The seed oil content per cent was 37, 40, 43 and 48% of canola plants treated with 0, 1, 1.5 and 2 mg Se L^–1, respectively. Total chlorophyll of canola was 1.94, 2.12, 2.35 and 2.63 mg g^-1 fresh weight of canola plants treated with 0, 1, 1.5 and 2 mg Se L^–1, respectively. While the proline was 4.45, 6.55, 6.95 and 7.35 μmol g^-1 fresh weight of canola plants treated with 0, 1, 1.5 and 2 mg Se L^–1, respectively. Total Se uptake by plants were 7.54, 206.87, 315.92 and 440.8 μg pot^-1_. While Se content in oil were 0.57, 15, 26.95 and 41.89 μg pot^-1 of canola plants treated with 0, 1, 1.5 and 2 mg Se L^–1, respectively.