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231262

ISOLATION AND CHARACTERIZATION OF GERANIUM (PELARGONIUM GRAVEOLENS) BACTERIAL ENDOPHYTES

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Botany

Abstract

ABSTRACT In the current study, eleven bacterial endophytes were isolated from the shoot system of aromatic medicinal plant Pelargonium graveolens .L. that inhabits North Sinai-Egypt . Out of eleven isolates, two identified on the basis of high nitrogen fixation as Bacillus subtilis (G5s) and Bacillus velezensis (G6s). The bacterial endophytic isolates showed varied activities as plant growth promoting agents. These two bacterial isolates have activity for ammonia production, siderophores and HCN. They exhibited enhanced qualitative Nitrogen fixation activity as well as quantitatively (nitrogenase assay) 365.667±1.8 C2H4/ml/24h and 87.767±1.6 C2H4/ml/24h respectively. They showed no significant assessment in phosphorus solubilization. The ability of the two isolates for producing phytohormone, indole-3-acetic acid (IAA) was detected using Salkowski's reagent and confirmed their productivity for IAA, gibberellic acid, abscisic acid, benzyl, kinetin, and ziaten by HPLC, G6s isolate exhibited maximum productivity of IAA with recorded 74.0 ± 2.1 mg mL–1 in the presence of 5 mg mL-1 tryptophan after 14 days. These two isolates had a high potential enzymatic activity for amylase, protease, cellulase, chitinase, and catalase except G6s that don't release amylase enzyme.

DOI

10.21608/ejas.2022.231262

Keywords

Key Words: bacterial endophytes, Geranium, biofertilization, nitrogen fixation, IAA

Volume

37

Article Issue

1

Related Issue

32205

Issue Date

2022-01-01

Receive Date

2022-06-16

Publish Date

2022-02-01

Page Start

31

Page End

57

Print ISSN

1110-1571

Link

https://ejas.journals.ekb.eg/article_231262.html

Detail API

https://ejas.journals.ekb.eg/service?article_code=231262

Order

4

Type

Original Article

Type Code

1,181

Publication Type

Journal

Publication Title

Egyptian Journal of Applied Science

Publication Link

https://ejas.journals.ekb.eg/

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-

Details

Type

Article

Created At

22 Jan 2023