ABSTRACT The impact of storage period and package light transmittance on retention of vitamin A and D3 content of fortified in pasteurized & sterilized cow''s milk, it can be indicated that packages without any light barrier properties like clear glass bottles are insufficient to protect the milk vitamins A and D3 under commercially relevant storage conditions. Due to the temperately short store ability of pasteurized milk, already the use of clear glass bottles led to a significant improvement so that after a 7day light-exposure period, losing 10% (90% retention) of the initial vitamin A and non-significant improvement of V D3 levels were losing 1.6% (98.4% retention), this means that vitamin D3 is more stability than vitamin A in light exposed under commercially relevant storage conditions and also package the milk in dark condition or high-pigmented bottle is better and led to more retention of both vitamin A and D3. In case of fortified sterilization milk protect from light exposed was necessary to obtain similar vitamins retentions of at least 88% under light expos and was been 94.1% in dark condition or protect bottles of vitamin A while vitamins D3 retentions reach 93.98% under light and 98.51% in dark of vitamins after a quite realistic light-exposure period of 60 days under commercially relevant storage conditions. However, it has to be considered that light-induced sensory changes in pasteurized milk and sterilization milk under commercially relevant storage conditions can only be excluded in light-tight packages.The stability of vitamin A and D3 was followed by its analysis in raw, pasteurized, and sterilized cow''s milk. Separation of vitamin A and D3 was done using C-18 column and isocratic elution of mobile phase consisting of 0.1 M acetic acid: acetonitrile (98: 2, v/v). Vitamin A and D3 were extracted from milk samples using 2.5 % solution of meta- phosphoric acid with extraction recoveries ranged 94 – 104 %. Confirmatory identification for presence of vitamin A and D3 in investigated milk samples were   achieved using UV and MS data obtained from reference standard and sample at the same retention time.