ABSTRACT Bio-ethanol is one of the energy sources that can be produced from renewable sources. Sugar beet wasteswere chosen as a renewable carbon source for ethanol fermentation because it is relatively inexpensive compared with other feedstock considered as food sources. However, saccharification processes are needed to convert cellulose of sugar beet wastes into fermentable or reducing sugars before ethanol fermentation. In this study, hydrolysis of sugar beet wastesand growth parameters of the ethanol fermentation were optimized to obtain maximum ethanol production by S. cerevisiae S288c. The ratio of plant material to acid solution of 1:10 (w/v). Results demonstrated that 0.5% H₂SO₄, 1% H₂SO₄,2% H₂SO₄ and 3% H₂SO₄ at 121ºC for 20 min by autoclave were enough to hydrolyze all cellulose contained in the Sugar beet wastes. The maximum yield of reducing or fermentable sugars was 124.80 mg/g obtained in 1% H₂SO₄. The minimum yield was 70.20 mg/g obtained in 3% H₂SO₄. The yield of bioethanol production by S. cerevisiae S288c was (50.96 g / 100 g) was achieved at pH 5.5, temperature of 30⁰C and inoculums size of 10% (v/v) after 72 hours of fermentation.