ABSTRACT The genus Yersinia belongs to the family Enterobacteriaceae and it constitutes facultative anaerobic, oxidase negative and catalase positive bacteria. Yersinia cells are Gram-negative straight rods or coccobacilli, carrying DNA with a guanine/cytosine content (the G+C content) of 46-50 mol %. Gastrointestinal disorders refer either to acute enterocolitis, particularly in children, or to pseudo appendicitis, in young adults In this study, pathogenic strains of Y. enterocolitica were identified by microbiological and PCR methods. The samples were collected from stool samples of children suffering from acute diarrhea. The techniques were used to isolate Y. enterocolitica from the samples PSB, and direct on the CIN, biochemical and PCR methods.PCR methods based on the identification of (YadA) virulence gene for detection, were used for the specific detection of the pathogenic strains of Y. enterocolitica. Traditional microbiological methods have been found to be insufficient for the specific identification of the Y. enterocoliticapathogen. Future research focusing on the identification using molecular methods or combining the two methods is necessary. The use of PCR has shown to be a sufficient standard of specific identification and is not time consuming.