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Antibiotic sensitivity and plasmid assessment of ampicillin-resistant <i>Vibrio cholerae</i> from seafoods in coastal areas of Ondo State

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial resistance

Abstract

Background:Vibrio choleraeis an epitome of non-invasive organisms, which only affects the small intestine via the liberation of enterotoxin.The plasmid profile and beta lactamase production in ampicillin-resistant Vibrio species isolated from seafoods collected from coastal area of Ondo State, Nigeria were investigated. Methods: One hundred and six (106) samples of seafoods were collected in February, 2020 and January, 2021. The isolation and identification of Vibrio species, antibiotic sensitivity pattern, plasmid analysis and beta-lactamase detection activity were conducted via pour plate, Kirby-Bauer disc diffusion, alkaline lysis, neutralization, electrophoresis methods, use of 10 % sodium dodecyl sulphate (SDS) and double disk synergistic test (DDST) respectively. Results: Pathogenic bacteria such as Bacillus subtilis, Enterobacter cloacae, Enterococcus faecalis, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus, and Vibrio cholerae were isolated from 10 crabs, 54 fishes, and 42 shrimps. Findings also revealed that 66% of Vibrio cholerae that showed multiple antibiotic resistance tested positive to beta lactamase production test. The result of plasmid analysis indicated that they were more susceptible to antibiotics after plasmid curing of the microorganisms. Conclusion: The resistance of Vibrio cholerae in this investigation was found to be plasmid-mediated consequent to its loss after curing. The detection of multidrug resistance, ESBLs-producing Vibrio organisms, suggests risks to public health.The risk of seafood-borne infections can be reduced by decrease in pollution levels, ensuring appropriate cooking temperatures, and prevention of cross-contamination during harvesting, handling and processing.

DOI

10.21608/mid.2022.113971.1224

Keywords

Seafoods, Vibrio cholerae, Beta-Lactamase, plasmid, antibiotics

Authors

First Name

Anthony

Last Name

Onifade

MiddleName

Kayode

Affiliation

Department of Microbiology, School of Life Sciences, Federal University of Technology, Akure

Email

profonifade@yahoo.com

City

Akure, Ondo State.

Orcid

-

First Name

Sawa

Last Name

Adeyanju

MiddleName

Joshua

Affiliation

Department of Microbiology, Federal University of Technology, Akure, Ondo State, Nigeria

Email

adeyanjusawa@gmail.com

City

Ondo

Orcid

0000000234461062

Volume

3

Article Issue

4

Related Issue

37291

Issue Date

2022-10-01

Receive Date

2021-12-31

Publish Date

2022-10-01

Page Start

1,006

Page End

1,015

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 3_Issue 4_Pages 1006-1015.pdf

PDF . 382.9kB

Link

https://mid.journals.ekb.eg/article_214513.html

Detail API

https://mid.journals.ekb.eg/service?article_code=214513

Order

27

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

Antibiotic sensitivity and plasmid assessment of ampicillin-resistant <i>Vibrio cholerae</i> from seafoods in coastal areas of Ondo State

Details

Type

Article

Created At

22 Jan 2023