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227004

Detection of virulence genes in Shiga toxigenic <i>Escherichia coli</i> isolated from diarrhoeic and non-diarrhoeic pediatric patients in Ondo State, Nigeria

Article

Last updated: 22 Jan 2023

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Subjects

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Tags

Medical bacteriology

Abstract

Background:Shiga toxigenic Escherichia coli (STEC) is one of the major causes of intestinal infection, bloody and non-bloody diarrhoea and extra-intestinal complications in humans. It is therefore necessary to enumerate the virulence genes present in isolated STEC strains. Methods: This research investigated the prevalence of STEC and identified some virulence genes (stx1, stx2, and eaeA) from diarrhoeic and non-diarrhoeic pediatric patients of age 1 to 16 years. A total of 243 stool samples were collected from diarrhoeic and non-diarrhoeic pediatric patients (in ratio 43:200) in four different State Specialist Hospitals in Ondo State, Nigeria. Polymerase chain reaction (PCR) assay was used for detection of Shiga toxin-producing E. coli. The virulence genes (stx1, stx2, and eaeA) were detected by PCR assay. Results: Out of the 243 samples, 163 (67%) were positive for E. coli and specifically 6 of them were positive for E. coli O157 (3.7%). Exactly 50% of the 6 E. coli O157 contained eaeA while all the strains contained Stx1 and Stx2. The results obtained also revealed that 6 strains that were non-O157:H7 STEC also contained Stx1 and four strains contained Stx2 genes. Conclusion: The results obtained from this study revealed that Stx1 and Stx2 genes are of the genes responsible for the virulence of STEC among diarrhoeic and non-diarrhoeic pediatric patients.

DOI

10.21608/mid.2022.124583.1252

Keywords

STEC, Shiga toxin, Virulence genes, Escherichia coli

Authors

First Name

Joy

Last Name

Olanrewaju

MiddleName

Efe

Affiliation

Department of Microbiology, Federal University of Technology, PMB 704, Akure, Nigeria

Email

efeolanrewaju@gmail.com

City

-

Orcid

-

First Name

Anthony

Last Name

Onifade

MiddleName

Kayode

Affiliation

Department of Microbiology, Federal University of Technology, PMB 704, Akure, Nigeria

Email

profonifade@yahoo.com

City

Akure, Ondo State.

Orcid

-

Volume

3

Article Issue

4

Related Issue

37291

Issue Date

2022-10-01

Receive Date

2022-02-28

Publish Date

2022-10-01

Page Start

998

Page End

1,005

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 3_Issue 4_Pages 998-1005.pdf

PDF . 370.3kB

Link

https://mid.journals.ekb.eg/article_227004.html

Detail API

https://mid.journals.ekb.eg/service?article_code=227004

Order

26

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

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Details

Type

Article

Created At

22 Jan 2023