Beta
ManageSearchChatDiscover
258248

Impact of Sofosbuvir and Daclatasvir therapy on the expression levels of inflammasomes in chronic hepatitis C infected patients

Article

Last updated: 22 Jan 2023

OverviewSimilar Items

Subjects

-

Tags

Medical virology

Abstract

Background and Aim:  Hepatitis C virus (HCV) infection is a leading cause of chronic hepatitis. Inflammasomes are multi-protein complexes that sense specific microbial molecules and trigger signaling cascades, leading to caspase1 activation and generation of pro-inflammatory cytokines, including IL-1β and IL-18. We aimed to investigate the expression levels of NLRP3, AIM2 and IFI16 inflammasome genes and serum levels of IL-18 in chronic HCV infected patients before treatment and after SVR12. Methods: The study included 30 chronic HCV infected patients and 30 healthy controls. The expression levels of inflammasome genes were evaluated by Quantitative real-time PCR (qPCR) and serum levels of IL-18 were evaluated by ELISA at baseline and after SVR12 with three months regimen of Sofosbuvir and Daclatasvir. Results: At baseline, the expression level of NLRP3, AIM2 and IFI16 inflammasome genes were higher in comparison to controls (p < /em> = 0.018, 0.000 and 0.155 respectively). In addition, the level of serum IL-18 was up regulated in comparison to controls (p < /em> = 0.000). After treatment, there was a statically significant decrease in the expression level of NLRP3, AIM2 and IFI16 inflammasome genes (p < /em> < 0.0001 for all). Also, there was a statically significant down regulation in the level of serum IL-18 (p < /em> = 0.000). Conclusion: Direct acting antiviral (DAA) therapy not only cause viral eradication but also has an immunological restitution effect as it decreases the expression level of NLRP3, AIM2 and IFI16 inflammasome genes and serum level of IL-18. This down regulation may decrease the risk of HCC development in chronic HCV infected patients.

DOI

10.21608/mid.2022.151668.1354

Keywords

NLRP3, AIM2, IFI16, IL-18, HCV

Authors

First Name

aya

Last Name

fergany

MiddleName

-

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Assiut University, Assiut, Egypt

Email

aya.fergany@yahoo.com

City

-

Orcid

-

First Name

Helal

Last Name

Hetta

MiddleName

-

Affiliation

Medical microbiology and immunology- faculty of medicine- Assuit university Internal medicine department, university of Cincinnati, USA

Email

helalhetta@aun.edu.eg

City

-

Orcid

-

First Name

Khaled

Last Name

Hassanein

MiddleName

-

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut, Egypt

Email

khaledhassanein70@yahoo.com

City

-

Orcid

-

First Name

Mohammed

Last Name

medhat

MiddleName

-

Affiliation

Tropical Medicine and Gastroenterology Department, Assiut University Hospitals, Assiut University, Assiut, Egypt

Email

mohammed.medhat@aun.edu.eg

City

-

Orcid

-

First Name

Gaber

Last Name

batiha

MiddleName

-

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicines, Damanhour University, Damanhour

Email

gaberbatiha@gmail.com

City

-

Orcid

-

First Name

Asmaa S.

Last Name

Shaltout

MiddleName

-

Affiliation

Faculty of Medicine, Medical Microbiology and Immunology Department, Assiut University, Assiut, Egypt

Email

asmaashaltout@aun.edu.eg

City

-

Orcid

-

Volume

3

Article Issue

4

Related Issue

37291

Issue Date

2022-10-01

Receive Date

2022-07-21

Publish Date

2022-10-01

Page Start

890

Page End

898

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 3_Issue 4_Pages 890-898.pdf

PDF . 408.0kB

Link

https://mid.journals.ekb.eg/article_258248.html

Detail API

https://mid.journals.ekb.eg/service?article_code=258248

Order

15

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023