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178421

Impact of dose and route of administration on antibody responses of chickens inoculated with inactivated Avian Influenza H5 vaccine.

Article

Last updated: 22 Jan 2023

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Subjects

-

Tags

Medical virology

Abstract

Background: The present study evaluated the influence of dose and route of administration of a commercial inactivated avian influenza virus (AIV) H5 vaccine on the humoral immune response of ISA brown chickens. Methods: Ninety “one-day-old" chickens were purchased from three commercial hatcheries (n = 30 chicks per hatchery), respectively, and chicks were vaccinated with either 0.2, 0.5 or 0.7 ml of the vaccine via either the intramuscular or subcutaneous route at days 14 and 28, respectively. Vaccinal antibody titres in chicks' sera were quantified using an indirect ELISA kit at 14 (before vaccination), 21, 28, 35 and 42 days of age. Results: Results showed significant differences (p < /em> < 0.001) in the mean antibody titre levels at day 21 of age between chicks from hatcheries C (2,205.0 ± 409.1) and A (57.7 ± 49.9) at 21 days of age when either 0.2 ml or 0.5 ml of the vaccine was administered IM or SC. In addition, there were intra- and inter dose significant differences ( p < /em>< 0.001) between the chicks at 21, 28, 35 and 42 days of age. Furthermore, intra- and inter route significant differences (p < /em>< 0.001) were detected between the chicks at 21 and 35 days of age. Conclusion:  Overall, the AIV H5 vaccine studied had variable outcomes and was poorly immunogenic. Recommendation: Further studies should be conducted to characterize the T- and B-lymphocytes in chickens post AIV H5 vaccines administration, and evaluate the sequence homologies between imported AIV H5 vaccines and circulating AIV strains in Nigeria.

DOI

10.21608/mid.2021.72759.1149

Keywords

Avian influenza, Inactivated H5 Vaccine, intramuscular, Subcutaneous, chickens

Authors

First Name

Abubakar

Last Name

Woziri

MiddleName

Ojone

Affiliation

Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria - Nigeria.

Email

woziriabubakar@gmail.com

City

Zaria

Orcid

0000-0001-6465-7704

First Name

Clement

Last Name

Meseko

MiddleName

Adebajo

Affiliation

Animal Influenza Division, Infectious and Transboundary Animal Diseases, National Veterinary Research Institute, Vom - Nigeria.

Email

cameseko@yahoo.com

City

Jos

Orcid

-

First Name

Faridah

Last Name

Nasir

MiddleName

Ibrahim

Affiliation

Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, Zaria - Nigeria.

Email

nasirfareedah@gmail.com

City

Zaria

Orcid

-

First Name

Khadijat

Last Name

Abdulkarim

MiddleName

-

Affiliation

Department of Zoology, Ahmadu Bello University, Zaria - Nigeria.

Email

wozirikhadijat@gmail.com

City

Zaria

Orcid

-

First Name

Folorunso

Last Name

Fasina

MiddleName

Oludayo

Affiliation

Emergency Centre for Transboundary Animal Diseases-Food and Agriculture Organization of the United Nations (ECTAD-FAO), Dar es Salaam, Tanzania.

Email

daydupe2003@yahoo.co.uk

City

Dar es salam

Orcid

-

First Name

Jibril

Last Name

Adamu

MiddleName

-

Affiliation

Department of Veterinary Microbiology, Ahmadu Bello University, Zaria - Nigeria.

Email

jiboadm@yahoo.com

City

Zaria

Orcid

-

First Name

Paul

Last Name

Abdu

MiddleName

Ayuba

Affiliation

Department of Veterinary Medicine, Ahmadu Bello University, Zaria - Nigeria.

Email

paabdu2004@yahoo.com

City

Zaria

Orcid

-

Volume

3

Article Issue

3

Related Issue

35766

Issue Date

2022-08-01

Receive Date

2021-05-04

Publish Date

2022-08-01

Page Start

733

Page End

743

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 3_Issue 3_Pages 733-743.pdf

PDF . 326.4kB

Link

https://mid.journals.ekb.eg/article_178421.html

Detail API

https://mid.journals.ekb.eg/service?article_code=178421

Order

26

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023