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A novel design of multi-epitope based vaccine against <i>Escherichia coli</i>

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Medical bacteriology
Microbial genetics and Proteomics

Abstract

Background: Multi-valent based vaccines have advantage over conventional vaccines because of its multi-faceted action targeted at antigen; thereby raising hope of a more sustained actions against allergens. Escherichia coli (E. coli) is a bacterium that is commonly found in the gut of humans and warm-blooded animals. An increasing number of outbreaks are associated with the consumption of fruits and vegetables (including sprouts, spinach, lettuce, coleslaw, and salad) thereby contamination may be due to contact with faeces from domestic or wild animals at some stages during cultivation or handling. Due to the reported increase in resistance to antibiotics used for Escherichia coli control; an effective vaccine is a would-be alternative of proven interest. Hence, a need for a rational, strategic, and efficient vaccine candidate against E.coli is of paramount necessity by the use of the most current bioinformatics tools to achieve this task. Method: In this study, immunoinformatics tools mined from diverse molecular databases were used  for a novel putative epitope based oral vaccine against E.coli. The prospective vaccine proteins were carefully screened and validated to achieve a high thorough-put three-dimensional protein structure. The eventual propsective vaccine candidate proteins was evaluated for its non-allergenicity, antigenicity, solubility, appropriate molecular weight testing and isoelectric point evaluation. Conclusion: The resultant vaccine candidate could serve as a promising anti-E.coli vaccine candidate. Immunoinformatics is a new field over pharmaco-therapeutics; this newest technology should continue to be a rescue from age-long traditional approach in vaccine developments.

DOI

10.21608/mid.2021.55310.1101

Keywords

Immunoinformatics, Allergenicity, Pharmaco-therapeutics, Multi-epitope, vaccine

Authors

First Name

Adeoti

Last Name

Micheal

MiddleName

O

Affiliation

Department of Science Laboratory Technology, Microbiology option, The Oke- Ogun Polytechnic, Saki Oyo State . Department of Pure & Applied Biology, Ogbomoso Ladoke Akintola University of Technology. Cellular Parasitology Unit, Department of Zoology, University of Ibadan, Nigeria.

Email

txy23m@yahoo.com

City

Ibadan

Orcid

-

First Name

Abdulwahab

Last Name

Jimoh

MiddleName

O

Affiliation

Department of Science Laboratory Technology, Microbiology option, The Oke- Ogun Polytechnic, Saki Oyo State Nigeria

Email

wabsonjimson@gmail.com

City

Ibadan

Orcid

-

First Name

Agoluaje

Last Name

Abiodun

MiddleName

Kabirat

Affiliation

Department of Science Laboratory Technology, Microbiology option, The Oke- Ogun Polytechnic, Saki Oyo State Nigeria

Email

agoluajeabiodun92@gmail.com

City

Ibadan

Orcid

-

First Name

A

Last Name

Agboluaje

MiddleName

K

Affiliation

Department of Science Laboratory Technology, Microbiology option, The Oke- Ogun Polytechnic, Saki Oyo State Nigeria

Email

-

City

-

Orcid

-

First Name

A

Last Name

Aderinto

MiddleName

O

Affiliation

Department of Science Laboratory Technology, Microbiology option, The Oke- Ogun Polytechnic, Saki Oyo State Nigeria

Email

-

City

-

Orcid

-

First Name

Taiwo

Last Name

Aliu

MiddleName

Sanusi

Affiliation

Department of Science Laboratory Technology, Microbiology option, The Oke- Ogun Polytechnic, Saki Oyo State Nigeria

Email

staiwo4ng@gmail.com

City

Ibadan

Orcid

-

First Name

D

Last Name

Adesina

MiddleName

A

Affiliation

Department of Microbiology and Botany, University of Ibadan, Ibadan, Nigeria

Email

-

City

-

Orcid

-

First Name

K

Last Name

Komolafe

MiddleName

A

Affiliation

Department of Science Laboratory Technology, Microbiology option, The Oke- Ogun Polytechnic, Saki Oyo State. Department of Zoology, Parasitology Unit, University of Ibadan, Nigeria

Email

-

City

-

Orcid

-

First Name

O

Last Name

Olaoye

MiddleName

J

Affiliation

Department of Science Laboratory Technology, Microbiology option, The Oke- Ogun Polytechnic, Saki Oyo State Nigeria

Email

-

City

-

Orcid

-

Volume

3

Article Issue

2

Related Issue

32908

Issue Date

2022-05-01

Receive Date

2020-12-26

Publish Date

2022-05-01

Page Start

407

Page End

419

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 3_Issue 2_Pages 407-419.pdf

PDF . 1.0MB

Link

https://mid.journals.ekb.eg/article_152416.html

Detail API

https://mid.journals.ekb.eg/service?article_code=152416

Order

20

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

A novel design of multi-epitope based vaccine against <i>Escherichia coli</i>

Details

Type

Article

Created At

22 Jan 2023