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223205

Evaluation of direct Carba-NP (CNPt) for screening of carbaenemases production in Gram negative bacilli

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Antimicrobial resistance

Abstract

Background: Carbapenem resistance among gram negative bacilli (GNB) is a major threat to human health. Rapid and accurate detection of carbapenemase production is very important for clinical management, epidemiological purposes and infection prevention and control issues. Direct Carba NP test (CNPt) is a modified CNPt that allows rapid detection of carbapenemases at a lower cost and improved sensitivity. This work aimed to evaluate direct-CNPt for detection of cabapenemases production as a screening and confirmatory test in GNB versus multiplex PCR as the gold standard test. Methods: This study was conducted on 50 clinical isolates of GNB derived from different clinical samples according to their resistance to meropenem (zone of inhibition ≤ 23 mm). Direct CNPt was performed on all 50 clinical isolates using bacterial colonies directly. A change in colour was observed after (̰ 2 h). A multiplex PCR was performed on all isolates to detect bla KPC, bla IMP, bla VIM and bla NDM, and bla OXA-48 genes. Results: The overall sensitivity and specificity of direct CNPt as compared to multiplex PCR were 95.2% and 100% respectively. The sensitivity to OXA-48 and IMP genes were relatively lower (91.6 % and 85.7 % respectively) than other genes which had 100% sensitivity for each. Conclusion: Direct CNPt is a reliable and rapid method that allows detection of different carbapenemases at a reduced cost. It could be used in combination with other phenotypic or genotypic assays in settings where OXA-48 and IMP are of high prevalence.

DOI

10.21608/mid.2022.119218.1243

Keywords

Carba NP test, phenotypic assay, Carbapenemase, real time PCR

Authors

First Name

Dina

Last Name

Erfan

MiddleName

-

Affiliation

Medical Microbiology and Immunology Department, Faculty of medicine, Ain Shams University, Egypt

Email

drdime03@yahoo.com

City

Cairo

Orcid

0000-0002-9742-4584

First Name

Walaa

Last Name

Ibrahim

MiddleName

-

Affiliation

Medical Microbiology and Immunology Department, Faculty of medicine, Ain Shams University, Egypt

Email

loulla_latif@yahoo.com

City

Cairo

Orcid

0000-0001-77627-8293

Volume

3

Article Issue

2

Related Issue

32908

Issue Date

2022-05-01

Receive Date

2022-02-03

Publish Date

2022-05-01

Page Start

378

Page End

386

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 3_Issue 2_Pages 378-386.pdf

PDF . 355.6kB

Link

https://mid.journals.ekb.eg/article_223205.html

Detail API

https://mid.journals.ekb.eg/service?article_code=223205

Order

17

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

Evaluation of direct Carba-NP (CNPt) for screening of carbaenemases production in Gram negative bacilli

Details

Type

Article

Created At

22 Jan 2023