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170293

Phenotypic detection of Metallo-β-Lactamase in imipenem-resistant <i>Pseudomonas aeruginosa</i> and <i>Stenotrophomonas maltophilia</i> at Schiphra Hospital of Ouagadougou in Burkina Faso

Article

Last updated: 22 Jan 2023

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Subjects

-

Tags

Medical bacteriology

Abstract

Background: Epidemic of carbapenemase-producing bacteria has become worldwide. Thus, during the last decade, the prevalence of carbapenem-resistant Enterobacteriaceae and non fermentative Gram-negative bacteria in human patients have increased. Carbapenemase-producing bacteria are usually multidrug resistant. Therefore, early recognition of carbapenemase producers is critical to prevent their spread. Objectives: The aim of this study was to contribute establishing the prevalence of isolates producing Metallo-β-Lactamase isolated from patients admitted to Schiphra Hospital of Ouagadougou. Methods: Susceptibility of bacteria to antimicrobial agents was evaluated by disc diffusion method using imipenem as screening antibiotic. The combination of imipenem-EDTA was used after detection the resistance to imipenem. Results: A total of 52 isolates resisting one of the third generation cephalosporins were collected. Five isolates showed an intermediary resistance to imipenem (9.61%). Two isolates were resistant to imipenem-EDTA (3.85%). The test of imipenem-EDTA was done to confirm to production of Metallo-β-Lactamase. The hydrolysis of bacterial extract by meropenem was confirmed by production with a kinetic activity at spectrophotometer V0=4.77x10–5µM/min for Pseudomonas aeruginosa and V0=1.183x10-4 µM/min for Stenotrophomonas maltophilia. Conclusion: This study showed that bacterial resistances by production to metallo-β-lactamases are a reality in Burkina Faso.

DOI

10.21608/mid.2021.70446.1138

Keywords

carbapenem, Imipenem-EDTA, Meropenem, Metallo-β-lactamases, Burkina Faso

Authors

First Name

Boukaré

Last Name

Kaboré

MiddleName

-

Affiliation

Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Burkina Faso, BP 7021 Ouagadougou 03

Email

kaboreboukare27@gmail.com

City

Ouagadougou

Orcid

-

First Name

Hama

Last Name

CISSE

MiddleName

-

Affiliation

Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Burkina Faso, BP 7021 Ouagadougou 03

Email

cissehama70@gmail.com

City

Ouagadougou

Orcid

0000-0001-7766-9501

First Name

Koudbi Jacob

Last Name

Zongo

MiddleName

-

Affiliation

Department of Biochemistry and Microbiology, Faculty of Applied Science and Technology, Burkina Faso, BP 176 DEDOUGOU

Email

zongo_kjacob@yahoo.fr

City

Ouagadougou

Orcid

-

First Name

Idrissa

Last Name

Sanou

MiddleName

-

Affiliation

UFR Health Sciences, University Joseph KI-ZERBO, Burkina Faso, BP 7021 Ouagadougou 03. Laboratory of Bacteriology and Virology at Tengadogo University Hospital, Ouagadougou, Burkina Faso, BP 104 Ouagadougou

Email

idrissasanou@yahoo.com

City

Ouagadougou

Orcid

-

First Name

Boukaré

Last Name

Zeba

MiddleName

-

Affiliation

Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Burkina Faso, BP 7021 Ouagadougou 03

Email

boubazeba@yahoo.fr

City

Ouagadougou

Orcid

-

First Name

Yves

Last Name

Traoré

MiddleName

-

Affiliation

Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Burkina Faso, BP 7021 Ouagadougou 03

Email

ytraore@yahoo.com

City

Ouagadougou

Orcid

0000-0003-2015-0068

First Name

Aly

Last Name

SAVADOGO

MiddleName

-

Affiliation

Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Burkina Faso, BP 7021 Ouagadougou 03

Email

alysavadogo@gmail.com

City

Ouagadougou

Orcid

0000-0002-9090-290X

Volume

3

Article Issue

1

Related Issue

29888

Issue Date

2022-02-01

Receive Date

2021-03-31

Publish Date

2022-02-01

Page Start

128

Page End

134

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 3_Issue 1_Pages 128-134.pdf

PDF . 386.6kB

Link

https://mid.journals.ekb.eg/article_170293.html

Detail API

https://mid.journals.ekb.eg/service?article_code=170293

Order

15

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023