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106549

Phenotypic detection of carbapenem resistant <i>Enterobacteriaceae</i> and characterization of <i>Klebsiella pneumoniae</i> carbapenemase(KPC) producing <i>Klebsiella pneumonia</i>

Article

Last updated: 22 Jan 2023

OverviewSimilar Items

Subjects

-

Tags

Infection prevention and control
Medical bacteriology

Abstract

Background: Carbapenem-resistant Enterobacteriaceae (CRE) pose a great threat to the public health sector as a result of its high communicability, high morbidity and mortality rates. This study investigated the occurrence of carbapenem resistant Enterobacteriaceae and the prevalence of Klebsiella pneumoniae carbapenemase (KPC) producing Klebsiella pneumoniae as a mechanism of resistance. Method: One hundred and ten Enterobacteriaceae isolates were cultured from different samples of patients in Intensive Care Units of two hospitals. Using Clinical and Laboratory Standards Institute (CLSI) Guidelines of 2017. Carbapenemase production was determined phenotypically using Brilliance CRE agar, Carbapenem inactivation method and confirmed with Modified Hodge test (MHT).  Modified Hodge test positive isolates were screened for KPC-producing Klebsiella pneumoniae using boronic acid-based inhibition test. Result: Phenotypic results revealed an occurrence of 10.9% (12/110) isolates for carbapenem resistant Enterobacteriaceae while KPC-producing Klebsiella pneumoniae prevalence was 3.6% contributing 33.3% to the burden of carbapenem production. The occurrence of carbapenem-resistant Enterobacteriaceae in this study was relatively high and KPC-Klebsiella pneumoniae had a significant contribution to the burden of CRE. Conclusion: Hence, a sinewy antibiotic stewardship is needed in this regard.

DOI

10.21608/mid.2020.36999.1040

Keywords

Carbapenem resistant enterobactereaceae, Modified hodge test, Boronic acid-based inhibition test, Klebsiella pneumoniae carbapenemase

Authors

First Name

Abidemi

Last Name

Ojo

MiddleName

Esther

Affiliation

department of Microbiology, College of Biosciences, Federal University of Agriculture, Abeokuta, Nigeria

Email

ojoae@funaab.edu.ng

City

-

Orcid

-

First Name

Seun

Last Name

Adebajo

MiddleName

Owolabi

Affiliation

Microbiology Department, College of Biosciences, Federal University of Agriculture, PMB 2240, Abeokuta, Ogun State, Nigeria.

Email

adebajoso@funaab.edu.ng

City

-

Orcid

-

First Name

kolapo

Last Name

Fasina

MiddleName

A

Affiliation

Biotechnology Division, Rubber Research Institute of Nigeria, PMB 1049, Iyanomo, Benin City, Edo State, Nigeria

Email

kolafasina@gmail.com

City

-

Orcid

-

First Name

Bolutife

Last Name

Odejinmi

MiddleName

-

Affiliation

Microbiology Department, College of Biosciences, Federal University of Agriculture, PMB 2240, Abeokuta, Ogun State, Nigeria.

Email

cutetifewealth04@gmail.com

City

-

Orcid

-

First Name

Olugbenga

Last Name

Ojo

MiddleName

anthony

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Madonna University, Port Harcourt, Rivers State, Nigeria

Email

ojoolugbenga46@gmail.com

City

-

Orcid

-

First Name

abiola

Last Name

Ooladotun

MiddleName

-

Affiliation

Microbiology Department, College of Biosciences, Federal University of Agriculture, PMB 2240, Abeokuta, Ogun State, Nigeria.

Email

oladotunat@funaab.edu.ng

City

-

Orcid

-

First Name

JOSHUA

Last Name

Ibeaha

MiddleName

-

Affiliation

Microbiology Department, College of Biosciences, Federal University of Agriculture, PMB 2240, Abeokuta, Ogun State, Nigeria.

Email

chrisjoe890@gmail.com

City

-

Orcid

-

Volume

2

Article Issue

4

Related Issue

28168

Issue Date

2021-11-01

Receive Date

2020-06-23

Publish Date

2021-11-01

Page Start

760

Page End

766

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 2_Issue 4_Pages 760-766.pdf

PDF . 261.6kB

Link

https://mid.journals.ekb.eg/article_106549.html

Detail API

https://mid.journals.ekb.eg/service?article_code=106549

Order

19

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023