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Identification of community-acquired methicillin-resistant <i>Staphylococcus aureus</i> (CA-MRSA) causing hospital-acquired infections in Suez Canal University Hospitals, Egypt by

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

Infection prevention and control
Medical bacteriology

Abstract

Background:Community acquired methicillin resistant staphylococcus aureus (CA-MRSA) is emerging in hospitals, worldwide. Method: In Suez Canal University Hospitals(SCUH), we documented CA-MRSA causing hospital acquired- (HA-) sepsis, by polymerase chain reaction (PCR) amplification of CA-MRSA major virulence determinant genes, namely: Panton-Valentine leukocidin "pvl", accessory regulator "agr" I and III, and staphylococcal cassette chromosome mec "SCCmec" genes IV and V. Antibiograms were determined by disk diffusion and minimum inhibitory concentration (MIC). Results: Methicillin resistant staphylococcus aureus  was detected in 90 hospital acquired infections (HAIs), including bacteremia, pneumonia, osteomyelitis, soft tissue infections, and meningitis. Pvl gene, characterizing CA-MRSA, was detected in 24/90 MRSA strains (26.7%). Pvl+ve strains were subtyped into SCCmec gene type II (8.3%), type IV (75%), type V (8.3%), and 8.3% were non-typeable.  They showed only agr group I (62.5%), and III (37.5%). Co-trimoxazole resistance was detected in 41.6% of CA-MRSA. Only 12.5% of CA-MRSA strains were susceptible to all non-beta-lactam drugs. There was no statistical correlation between SCCmec or agr groups, and co-trimoxazole resistance in CA-MRSA; nor between SCCmec types and agr groups. Conclusion: Community aquired-MRSA is emerging in all wards of SCU hospitals, causing HAI, mostly soft tissue infections. The classical antibiogram of CA-MRSA is no longer prevailing. Diagnosis of CA-MRSA should rely upon detection of pvl gene, rather that clinical and antibiogram-profiles. The name "CA-MRSA" is no longer satisfactory to describe such strains in hospital settings; instead, Pvl +ve MRSA is more accurate and reliable term to use.

DOI

10.21608/mid.2020.41062.1057

Keywords

CA-MRSA, agr and sccmec typing of CA-MRSA, hospital CA-MRSA

Authors

First Name

Mohamed

Last Name

Al sweify

MiddleName

A

Affiliation

Suez Canal University, Faculty of Medicine, Microbiology and Immunology Department, Ismailia, Egypt

Email

msweify@yahoo.com

City

Ismailia

Orcid

-

First Name

Atef

Last Name

Raheel

MiddleName

-

Affiliation

Suez Canal University, Faculty of Medicine, Microbiology and Immunology Department, Ismailia, Egypt

Email

atefraheel@yahoo.com

City

Ismailia

Orcid

0000000246626629

First Name

Hassan

Last Name

Aboul-Atta

MiddleName

-

Affiliation

Suez Canal University, Faculty of Medicine, Microbiology and Immunology Department, Ismailia, Egypt

Email

aboulatta_hassan@hotmail.com

City

Ismailia

Orcid

-

First Name

Gehan

Last Name

El-Hadidy

MiddleName

-

Affiliation

Suez Canal University, Faculty of Medicine, Microbiology and Immunology Department, Ismailia, Egypt

Email

gehan-sh@hotmail.com

City

Ismailia

Orcid

-

First Name

Waheed

Last Name

Hessam

MiddleName

-

Affiliation

Suez Canal University, Faculty of Medicine, Microbiology and Immunology Department, Ismailia, Egypt

Email

w_hessam@hotmail.com

City

Ismailia

Orcid

-

Volume

2

Article Issue

4

Related Issue

28168

Issue Date

2021-11-01

Receive Date

2020-07-04

Publish Date

2021-11-01

Page Start

715

Page End

724

Print ISSN

2682-4132

Online ISSN

2682-4140

Link

https://mid.journals.ekb.eg/article_118067.html

Detail API

https://mid.journals.ekb.eg/service?article_code=118067

Order

15

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

Identification of community-acquired methicillin-resistant <i>Staphylococcus aureus</i> (CA-MRSA) causing hospital-acquired infections in Suez Canal University Hospitals, Egypt by detection of its major virulence determinants

Details

Type

Article

Created At

22 Jan 2023