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167399

Molecular detection of bacterial agents of atypical pneumonia among patients from six hospitals in Suez Canal region

Article

Last updated: 22 Jan 2023

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Subjects

-

Tags

Medical bacteriology

Abstract

Background: Reliable diagnosis of atypical pneumonia microbial agents is crucial since they do not respond to beta-lactams but to other groups of antibiotics. Therefore, empiric treatment with beta lactam groups will be ineffective for their eradication if no additional antibiotics as macrolides are administered to the management plan. This study aimed to determine prevalence of Mycoplasma pneumoniae (M. pneumoniae), Chlamydophila pneumoniae (C. pneumoniae) and Legionella pneumophila (L. pneumophila) among patients with atypical pneumonia, and drawing physicians' attention to the role of these pathogens as etiologic agents of atypical pneumonia in the Suez Canal region. Methods: Eighty-four atypical pneumonia cases were enrolled in this study. Respiratory samples were collected. Part of each specimen was inoculated onto blood, MacConkey, and chocolate agar plates, another part of specimens was processed for DNA extraction and multiplex PCR assay for detection of M. pneumoniae p1adhesion gene, C. pneumoniae outer membrane protein (ompA) gene, and L. pneumophila macrophage infectivity potentiator (mip) gene. Results: Out of the 84 atypical pneumonia cases, atypical bacteria were detected by multiplex conventional PCR in 12 (14%) cases and they all were L. pneumophila, 3 cases (4%) were mixed with Staphylococcus aureus, and 2 (2%) cases were mixed with Streptococcus pyogens. Mycoplasma pneumoniae and C. pneumoniae were not detected by PCR in our samples. Conclusions: Legionella  pneumophila incidence is not low in our geographical region in patients with atypical pneumonia; so it is of pivotal importance to recruit sensitive and reliable molecular based techniques to detect and control this infection in healthcare environments.

DOI

10.21608/mid.2021.69228.1135

Keywords

Atypical pneumonia, Legionella pneumophila, mip gene, Suez Canal region, Egypt

Authors

First Name

Heba

Last Name

El-Sayed

MiddleName

-

Affiliation

Department of Microbiology, and Immunology, Faculty of Medicine, Port-Said University, Port-Said, Egypt

Email

dr.heba286@gmail.com

City

Port-Said

Orcid

-

First Name

Mohamed

Last Name

Al sweify

MiddleName

Ali

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.

Email

msweify@yahoo.com

City

Ismailia

Orcid

-

First Name

Waheed

Last Name

Hessam

MiddleName

-

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.

Email

w_hessam@hotmail.com

City

Ismailia

Orcid

-

First Name

Eman

Last Name

Hamed

MiddleName

Riad

Affiliation

Internal Medicine Department ,Chest Unit, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Email

emanriad@med.suez.edu.eg

City

Ismailia

Orcid

-

First Name

Marwa

Last Name

Fouad

MiddleName

-

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.

Email

marwafouad80@gmail.com

City

Ismailia

Orcid

0000-0002-3883-2916

Volume

2

Article Issue

3

Related Issue

26240

Issue Date

2021-08-01

Receive Date

2021-03-23

Publish Date

2021-08-01

Page Start

575

Page End

585

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 2_Issue 3_Pages 575-585.pdf

PDF . 382.2kB

Link

https://mid.journals.ekb.eg/article_167399.html

Detail API

https://mid.journals.ekb.eg/service?article_code=167399

Order

23

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023