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181903

Detection of plasmid-mediated colistin resistance in carbapenem-resistant Escherichia coli and Klebsiella pneumoniae isolates in Suez Canal University Hospitals

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Medical bacteriology

Abstract

Background: The polymyxins including colistin are the “last resort" antibiotics for treatment of infections with carbapenem resistant Enterobacteriaceae. Recently, transferable colistin resistance mediated by the mcr-genes has been described in Enterobacteriaceae. The Testing of colistin susceptibility is challenging. The aim of the work is to detect the occurence of mcr-1 and mcr-2 genes in phenotypically colistin-resistant carbapenem resistant Escherichia coli and Klebsiella pneumoniae isolates using conventional PCR. Methods: One hundred and sixteen carbapenem-resistant E.coli and K.pneumoniae isolates were collected from patients admitted to different wards of Suez Canal University Hospitals in Ismailia. Urine, endotracheal aspirates, blood, pus and sputum specimens were collected from different patients. Minimal inhibitory concentration by broth microdiluton method was done to assess phenotypic colistin resistance. The colistin resistant E.coli and K.pneumoniae isolates were tested by conventional PCR to detect plasmid mediated mcr-1 and mcr-2 genes. Chi-square test was applied and p < /em>-value < 0.05 was considered statistically significant. Results: Forty three isolates out of 116 carbapenem resistant isolates (37.1%) were colistin resistant as shown by MIC by broth microdiluton method. The 43 colistin resistant E.coli and K.pneumoniae isolates were tested by conventional PCR to detect plasmid mediated mcr-1 and mcr-2 genes. Two mcr-2 genes and one mcr-1 gene were detected. Conclusion: Results revealed that the prevalence of colistin resistance among carbapenem-resistant E.coli and K.pneumoniae in SCUHs is concerning; further limiting potential therapeutic options. Plasmid mediated colistin resistance genes mcr-1 and mcr-2 is emerging in SCUHs which refer to a problem in the hospital as by horizontal transfer of this plasmid, the resistance can spread to many isolates in the hospital.

DOI

10.21608/mid.2021.78200.1162

Keywords

PCR, Enterobacteriaceae, Colistin, resistance

Authors

First Name

sally

Last Name

Khattab

MiddleName

-

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.

Email

sally.maged@med.suez.edu.eg

City

Ismailia

Orcid

0000-0001-7731-4962

First Name

Mohamed

Last Name

Al sweify

MiddleName

Ali

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.

Email

msweify@yahoo.com

City

Ismailia

Orcid

-

First Name

lobna

Last Name

metwally

MiddleName

-

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt. Microbiology department, College of Medicine, Taif university

Email

lobna.metwally@gmail.com

City

Ismailia

Orcid

-

First Name

Sahar

Last Name

Elazab

MiddleName

-

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.

Email

saharmicrobiology@yahoo.com

City

Ismailia

Orcid

-

First Name

Asmaa

Last Name

Hashem

MiddleName

-

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.

Email

asmamicro82@gmail.com

City

Ismailia

Orcid

-

Volume

2

Article Issue

3

Related Issue

26240

Issue Date

2021-08-01

Receive Date

2021-06-02

Publish Date

2021-08-01

Page Start

497

Page End

507

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 2_Issue 3_Pages 497-507.pdf

PDF . 492.3kB

Link

https://mid.journals.ekb.eg/article_181903.html

Detail API

https://mid.journals.ekb.eg/service?article_code=181903

Order

15

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

Detection of plasmid-mediated colistin resistance in carbapenem-resistant Escherichia coli and Klebsiella pneumoniae isolates in Suez Canal University Hospitals

Details

Type

Article

Created At

22 Jan 2023