Background: Antibiotic resistance is a challenge to treat. Extended Spectrum Beta Lactamases (ESBL) is one of the phenomenon by which the uropathogen express resistance to the commonly used antibiotic. Objectives: To determine the frequency of ESBL in uro-pathogens and ESBL preference for age and gender. Methods: This cross sectional study was conducted  in Qazi Hussain Ahmed Medical Complex Nowshera from 1^stMar 2019 to 31^st Jan 2020. A total of 315 urine samples were received for Culture and sensitivity out of which 77 cases reported to be ESBL positive (24.44%).Relevant information's were recorded in SPSS version 25. Results: A total of 77 cases with established ESBL phenomenon were studied. 40% were males and 60% females. The frequency of ESBL positive isolates was (24.44%). In 71(92%) cases of ESBL , Escherichia coli strain was reported, Klebsiella pneumonia in 5(6.5%) and Proteus mirabilis in 1.3%  cases. The difference in Means +SD of age of the male gender (24.25years+9.6) was statistically significant with female gender(31.16years+14.49) (p-value 0.02). The probability of ESBL infections was 2.16 times higher in female gender (p-value: 0.01, OR:2.16). The sensitivity pattern of the ESBL producing E. coli was;  Imipenem(IMP) 100%, Meropenem(Mem)100%, Piperacillin Tazobactam(TZP)  92%, Nitrofurantoin (F)-85% . While Imipenem & meropenem were drugs of choice for ESBL-Klebsiella pneumonia and ESBL -Proteus mirabilis. Conclusion: The frequency of ESBL isolates was 24.44%. Female gender reported more ESBL infection as compared to male gender. Escherichia coli ESBL was most common. The common sensitivity pattern to ESBL was: Imipenem(IMP) 100%, Meropenem(Mem)100% and Piperacillin Tazobactam 95%.