Background: Somebacteria possess fimbriae that support their adherence to host cell surface, colonization of human epithelial cells and resistance to antibiotics. Aim: The expression of type 1 fimbriae and antibiotic resistance by isolates from HIV infected patients. Methods: Bacteriological analysis of mid-stream urine (MSU) samples of human immunodeficiency virus infected patients (n=61) was determined using microbiological technique. In vitro type 1 fimbriae expression and antibiotic susceptibility of bacteria was determined using human erythrocytes with D-mannose and disc diffusion technique. Results: Of the 61 MSU samples from subjects, 31.1% had positive bacterial growth ≥ 10⁵ CFU/mL. Forty-nine (49) bacterial isolates, belonging to genera Staphylococcus, Escherichia, Pseudomonas, Streptococcus, Klebsiella, Enterococcus and Proteus were recovered from the MSU samples. Escherichia coli was predominant isolate with 26.5%, followed by S. aureus (18.4%), while E. faecalis had the lowest occurrence (4.1%). Of the 29 isolates exhibiting hemagglutinating activity, type 1 fimbriae was expressed by 16 isolates, while 13 isolates showed P-fimbriae. The results showed 18.4 % isolates exhibiting resistance to Gentamycin; between 30.6 % and 38.8% isolates were Tetracycline and Nitrofurantoin resistant, while ≤ 44.9% isolates were resistant to Chloramphenicol and Ampicillin. Proteus sp had a low level of resistance to Ciprofloxacin, while ≥ 46.2 % E. coli were resistant to Nitrofurantoin. There was significant relationship (p < 0.05) between isolates expressing type 1 fimbriae and resistance to Gentamycin, Nalidixic Acid and Ampicillin. Conclusion: This study has shown the phenotypic expression of type 1 fimbriae and antibiotic resistance by isolates from HIV infected patients.