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100635

Comparison of different techniques and stains for direct diagnosis of Trichinella spiralis

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

Medical parasitology

Abstract

Background: The aim of Trichinella inspection is to reliably detect larvae in meat at levels which are capable of causing human trichinellosis. Trichinoscopy is a rapid, cost effective but is less sensitive in detection of low muscle larvae (ML)numbers. Artificial digestion is more convenient, and flexible, but may destroy immature larvae. Baermann technique may enhance the efficiency of diagnosis. Giemsa and Leishman stains provide identical contrasting coloration as Hematoxylin and Eosin (H-E) with the both advantages of that they can be used for staining non-histological sectionsand rapid preparation.The primary aim of the study was to compare the efficiency of digestion and Baermann techniques for detection of ML in muscles. Secondary aim was detection of ML by Geimsa and Leishman stain and their comparison with routinely used stain H-E. Methods: Muscles from 36 mice orally infected with Trichinella spiralis encysted larvae were examined. Larvae from 1 g samples of different muscles were recovered by digestion and Baermann techniques after different dose of infection. Muscle samples were prepared for staining with Giemsa, Leishman and H-E. Results: Number of larvae recovered by Baermann was higher recovered by digestion method (P ˃0.5). Leishman stain was the best stain for rapid detection of ML after 30 min followed in validity by Geimsa then H-E. Conclusion: Baermann concentration technique showed higher sensitivity than digestion method. Leishman stain is superior to Giemsa stain as it takes less time. Both stains are considered good alternative to H-E for rapid and easy diagnosis of trichinellosis in post-mortem animals.

DOI

10.21608/mid.2020.33288.1026

Keywords

Baermann method, digestion method, experimental trichinosis, Giemsa stain, leishman stain

Authors

First Name

Eman

Last Name

Mostafa

MiddleName

-

Affiliation

Parasitology Department , Faculty of Medicine , Zagazig University, Zagazig, Egypt.

Email

emanmostafa81@gmail.com

City

zagazig

Orcid

-

First Name

shimaa

Last Name

Hassan

MiddleName

Samir

Affiliation

Zoology Department , Faculty of Science, Zagazig University.Egypt

Email

sh_samir2009@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Ahmed

MiddleName

-

Affiliation

Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt

Email

mohsamir2016@yahoo.com

City

-

Orcid

-

Volume

1

Article Issue

3

Related Issue

16205

Issue Date

2020-11-01

Receive Date

2020-06-23

Publish Date

2020-11-01

Page Start

199

Page End

208

Print ISSN

2682-4132

Online ISSN

2682-4140

Link

https://mid.journals.ekb.eg/article_100635.html

Detail API

https://mid.journals.ekb.eg/service?article_code=100635

Order

17

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

Comparison of different techniques and stains for direct diagnosis of Trichinella spiralis

Details

Type

Article

Created At

22 Jan 2023