Athletic competition is an especial occasion with formal framework requires athletes to exert maximum effort. This study aims profiling the psychophysiological impact of official handball match on salivary cortisol levels and immunoglobulin IgA as detectors of body reaction to match physiological demands and how players can react psychologically and be reflected in their physiological responses. Descriptive curriculum carried out matching the aim of the study, Sample was collected intentionally from Tanta handball team, it is a tenth degree in Egyptian handball league, 16 elite handball players participated in this study, (4 pivots, 4 wings, 4 goalkeepers, 4 backcourts), with average age of 21.625, weight of 77.250 and heart rate of 93.500. Measurements were divided into two tests, one is a psychological test with self-check questionnaire, to detect the stress of competing before match, and the other one is salivary cortisol and immunoglobulin IgA levels. Goal keepers, back court, pivot and wings groups recorded insignificant differences in anxiety level pre-match up, however, related reading between cortisol and IgA levels. Cortisol and IgA levels were different among the groups; the higher reading was for goal keepers, and then back courts and pivot groups, wings group recorded the lowest cortisol and IgA levels, that explained the impact of the playing position on the plyers' bodies, as well as emphasized on the playing experience as an adaptive agent manipulating the psycho-physiological responses.