Aim: The present study aimed at developing nurses` performance guidelines for patients undergoing cholecystectomy based on needs assessment. Methods: A descriptive exploratory design was utilized for the conduction of this study. This study was conducted in the surgical departments at El demerdash surgical Hospital, affiliated to Ain Shams University. Sample: A convenient sample of 50 nurses from both genders in the surgical wards working with patients undergoing (open and laparoscopic) cholecystectomy. They were recruited from the above-mentioned settings. Tools: 1) Self-administered questionnaire which include sociodemographic data and nurses` knowledge assessment. 2) An observational checklist.  Results: The mean age of the studied nurses was 43.25 ± 11.01. There was a highly positive relation between studied nurses' total levels of practices and knowledge. There was a statistically significant relation between nurses` knowledge and practices as regards their demographic characteristics. Conclusion: Nearly two thirds of the studied nurses had unsatisfactory level of knowledge and practices in relation to caring of patients undergoing cholecystectomy. On the same line a statistically significant relation was indicated as regards their demographic characteristics and cholecystectomy surgery.  Recommendations: Further research studies are needed to identify the effect of implementing the developed guidelines on performance of nurses caring for patients undergoing cholecystectomy.