Vicia faba, also referred to as the broad bean, is a plant that is extensively grown for human food. It can be eaten both raw and cooked. Alternaria leaf spot is caused by the alternate occurring late in the growing season, which can severely impact yield. In this experiment six isolates of A. alternata were isolated from diseased natural fields of Vicia faba plants from different areas in the Qena governorate, identified according to their morphological characters as A. alternata.  The effect of biological agents Trichoderma harzianum and Bacillus subtitles and natural plants extract garlic (Allium sativum) and neem (Azadirachta indica), used in the concentration of 5% 10% and 15%, respectively were examined in the laboratory for their antagonistic effect against A. alternata.  Trichoderma harzianum had a 71% inhibition percent for the pathogenic linear growth and Bacillus subtitles had 58%. Natural plants extract garlic and neem were more effective and could suppress 90 and 100% respectively. Natural plants extract were more effective in reduce linear growth of A. alternate isolated from vicia faba compared with Trichoderma harzianum and Bacillus subtitles.