Pollen is one of the bee-hive products, which it's rich in protein and a wide range of vitamins. A study was carried out to evaluate the efficiency of pollen as nutritive supplementation on silkworm Bombyx mori Land compare its effect with ascorbic acid and an antibiotic (tetracycline). Three concentrations (2, 5 and 10 %) from (pollen, ascorbic acid, and tetracycline) were sprayed on fresh mulberry leaves and fed to silkworms, from the 4^th to 5^th instar larvae. The study was conducted to find out the physiological and biochemical changes in the haemolymph that occurred in the 5^th instar larva. Some physiological parameters such as T.H.S, T.H.C, and D.H.T and six biochemical parameters such as total protein, total carbohydrates, total lipids, ALP, GOT and GPT, have been correlated with some productive traits as larval weights and cocoons parameters. The results showed pollen's ability to improve the physiological and economical characteristics of silkworm. Pollen at a concentration of (5%) had the best results. Further, the results suggest that coadministration of pollen (5%) with mulberry leaves has enhanced the biochemical reaction involved in the silk production in the silkworm.