This study aimed to investigate the effects of green Nano-Copper oxide (Nano-CuO) on growth performance, and serum biochemistry of broiler chickens. A total of 96One-day old broiler chickens were randomly distributed into two equal treatment groups. Treatment groups were fed a control diet or a control diet supplemented with green synthesis of Nano-CuO (8 mg/kg).  The feeding trial lasted for 35 days. Each treatment had six replicates with eight birds each. The diets were formulated to meet Ross 308 broiler recommendations. Chicks were full access to feed and water during the experimental period. The brooding temperatures were 38.5, 36.5, and 30.9°C during 1–10, 10–21, and 22–42 d of age, respectively. The results showed that body weight gain and feed conversion ratio were significantly improved Nano-CuO treated group than non-supplemented group. Furthermore, carcass characteristics were significantly improved (P<0.01) in Nano-CuO compared to control groups. Likewise, supplementation of Nano-CuO significantly improved liver and kidney functions as indicated from serum metabolites. Therefore, it can be concluded that Nano-CuO, achieved sustainable development in broiler production under climate change challenges.