This experiment was carry out for two successive seasons on Flame seedless grapevines (2019 & 2020) in vineyard of EL-Baramon experimental farm, Horticultural Research Institute, Mansoura, Egypt. The target of this present study was identify the optimum planting distance and number of buds which must be left per square meter to gain the best results with respect to bud behavior, characteristics of vegetative growth ,  yield, canopy microclimate and quality of berries. The vines were planted under three different levels of planting distances (1.5 x 3m & 2  x 3m and 2.5  x 3m) and were loaded at three different levels of buds load (8 &10 and12 buds/ m²). Results showed that all studied characteristics were significantly affected with planting distance and load of buds. Increasing planting distance from (1.5m×3m) to (2.5m×3m) was accompanied with an improve in vegetative growth parameters, nutritional status, yield/vine, microclimatic data and chemical properties in berries, while decreased bud burst%, cluster and berry weight yield/ feddan. Also, increase load of buds/vine from (8 buds/ m²) to (12 buds/ m²) was accompanied by an increase in yield/vines and feddan, while negatively effected on bud burst%, vegetative growth parameters, microclimatic data, chemical properties in berries and total carbohydrates in cans.It could be suggested that planting Flame seedless grapevines at planting distance (2.5mx3m) or (2mx3m) with retained (8buds/m²) were considered the most optimum treatment for improving vines nutritional status, vegetative growth characteristics, microclimatic data and quality of berries.