This study was conducted during 2018, 2019 and 2020 seasons, on 10 years old Flame Seedless grapevines, grown on the experimental vineyard of Research Station Agriculture, EL-Matana, Esna, Luxor, Egypt to study the effect of organic and bio-fertilization on vegetative growth, nutrient status, and fruiting of Flame Seedless grapevines. The experiment was arranged in a complete randomized block design with seven treatments and three replications two vine per each. The obtained results could be summarized as follow: Using the recommended dose of nitrogen (RDN) via 50 or 25% mineral plus 50 or 75% or 50% organic and bio-form significantly increased, pruning wood weight, number of leaves/shoot and leaf area as well as leaf total chlorophyll and leaf nutrient composition compared to use RDN via mineral N fertilizer alone. No significant differences on these traits due to use RDN via 50% mineral plus 50 organic or bio, double form or 25% N plus 75 bio-form and triple born. All combined fertilization treatments significantly increased the yield and improved the cluster and berry traits compared to use RDN via mineral source only. It is evident from the foregoing results that double form 50% mineral plus 50% either organic or bio or triple form (25% N plus, 75 organic and bio.) improved the vegetative growth, yield and berry quality. In addition, it minimized the production costs and reduced environmental pollution.